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Functional validation of sorted fractions was performed by xenotransplantation, the results of which was combined with miRNA expression profiling to make stem cell-similar miRNA expression profiles.
To find out no matter whether miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML client samples and three lineage-depleted (Lin–) twine blood (CB) samples using CD34 and CD38 into four populations and subjected each to world wide miRNA profiling; the stem cell articles of each and every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation measured by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was lowered in two of three samples upon 126KD (Figures 5C and S5D). Together, these findings suggest that 126KD produces heterogeneous responses with LSC function and frequency reduced in a subset of AML people.
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(E) Percent BrdU incorporation into bulk cultures demonstrating proliferation of CTRL and 126OE transduced 8227 cells over time.
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